Biocompatibility of type I sponge collagen with rabbit's adipose-derived stem cellsin vitro

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Abstract

BACKGROUND:

It is important to search a type of scaffolds to be served as carriers of adipose-derived stem cells (ADSCs) culture.

OBJECTIVE:

To study the biocompatibility of type I sponge collagen protein and ADSCs.

DESIGN, TIME AND SETTING:

An in vitro observation was performed at the Research Center of Tissue Engineering of the Southern Medical University from January to March in 2007.

MATERIALS:

Eight female New Zealand rabbits, with 3-4 months years were supplied by experimental animal center of Southern Medical University. The type I sponge collagen was provided by Guangzhou Trauer Biotechnology Co., Ltd.

METHODS:

Subcutaneous fat were cut from anesthetized rabbits, the 3 passages of ADSCs were isolated and regulated to obtain 1×109/L cell suspension. The aseptic type I collagen sponge was cut into 10 mm×10 mm×5 mm pieces and put into 96-well plate, vaccinated 0.1 mL cell suspension, and co-cultured with DMEM with fetal bovine serum.

MAIN OUTCOME MEASURES:

Morphology and expressions of the surface markers, cells adhesion and proliferation on the scaffolds, as well as the compound of co-cultured type I sponge scaffold with ADSCs were observed by inverted phase contrast microscope and scanning electron microscope.

RESULTS:

The primary ADSCs mainly wide, applanate and short fusiform shape, which was fusiform shape after passaged. The 3 passage of ADSCs were positive expressed CD29 and CD 44. In addition, the average adherence rate of co-culture 3 passage ADSCs with type I collagen sponge scaffold was 92.38%, which remained a normal cells proliferate rate. Under light microscope, more adherent cells could be found as increasing time of culture, and red fluorescence could be seen at the cell membrane after DiI staining. Parts of cells grew in clumps and distributed aggregative, attached to the surface and pore space of sponge, with plenty of matrix under electron microscope.

CONCLUSION:

Type I collagen sponge scaffolds have a good biocompatibility with ADSCs of rabbits, which can be used as a vehicle for seeding cells of adipose tissue engineering.

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