Recent reports indicate that enzyme-electrode glucose biosensor is limited by glucose-oxidase deactivation, poor stability and repeatability; therefore, glucose biosensor characterizing by rapid, stable, and exact arouses more and more attention.OBJECTIVE:
To construct a novel electrochemical glucose biosensor.DESIGN, TIME AND SETTING:
An observational experiment was performed at Bisensor Laboratory of Department of Medicine Laboratory, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA from August 2008 to February 2009.MATERIALS:
The modified electrode was constructed, based on immobilization of glucose oxidase (GOD) on an Au electrode by electrostatic effect, which was modified with MWCNTs by utilizing self-assemble techniques and NanoAu by electrodeposition.METHODS:
The ferricyanatum Kalium was used as probe. The properties of glucose biosensor were characterized by cyclic voltammetry (CV) and linear scan voltammetry (LSV) in the three-electrode system. Relation between glucose concentration and response peak current about different modified electrode were compared.MAIN OUTCOME MEASURES:
The cyclic voltammetry of different modified electrodes; relation between glucose concentration and response peak current.RESULTS:
Primary study indicated that the biosensor exhibited excellent performances for glucose detection with a low detection limit (0.4 μmol/L), a wide linear range (0.01-10 mmol/L), a fast response time (< 2 s), and good reproducibility and stability. In addition, the biosensor was applied in the determination of glucose in human blood samples and the results which were obtained from biosensor were agreement with Hexokinase (HK).CONCLUSION:
The biosensors exhibit good reproducibility, stability and fast response to glucose, so it can be used in the clinic detection of glucose concentration in human serum.