Effect of adriamycin nanoparticles conjugated with hyaluronic acid on oral squamous cell carcinomasin vitro*⋆

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Abstract

BACKGROUND:

Adriamycin (ADM) can specifically conjugate with receptor. In particular, ADM nanoparticles play a target role in decreasing the cytotoxicity.

OBJECTIVE:

To investigate the targeting effect of adriamycin nanoparticles conjugated with hyaluronic acid (ADM-HA-SSL) on oral squamous carcinomas cells in vitro.

DESIGN, TIME AND SETTING:

An in vitro contrast observation was performed in College of Marine Life Science, Ocean University of China from January to July 2008.

MATERIALS:

Oral squamous carcinomas cells were sincerely presented by Professor Chen from the Ninth People's Hospital of Shanghai; ADM-HA-SSL (drug loading 156 mg/L) was sincerely presented by Professor Liu from College of Marine Life Science, Ocean University of China.

METHODS:

Oral squamous carcinomas cells were cultured with 0.5, 1.0, 5.0, and 10.0 mg/L ADM-HA-SSL. MTT assay was used to detect the targeted cytotoxicity of ADM-HA-SSL against oral squamous cell carcinomas. With the concentrations of 5.0 and 10.0 mg/L, cell apoptosis was ascertained by cell flow cytometry after 6, 12, 24 and 48 hours.

MAIN OUTCOME MEASURES:

Cell survival rate and apoptosis rate.

RESULTS:

At 24 and 48 hours after induction, cytotoxicity assay revealed that the effect of ADM-HA-SSL was superior to that of free ADM (t=5.78−42.05, P < 0.01). The results of flow cytometry showed that the apoptosis rate was enhanced with the increase of the time (F=4 200.40, 4 775.36, P < 0.01), and the rate was also increased at the same time point with the increase of concentration (t=12.06-20.08, P < 0.05).

CONCLUSION:

ADM-HA-SSL can specifically recognize oral squamous carcinomas cells and deliver adriamycin into the cells. And the effect is enhanced by the time prolonging.

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