Adriamycin (ADM) can specifically conjugate with receptor. In particular, ADM nanoparticles play a target role in decreasing the cytotoxicity.OBJECTIVE:
To investigate the targeting effect of adriamycin nanoparticles conjugated with hyaluronic acid (ADM-HA-SSL) on oral squamous carcinomas cells in vitro.DESIGN, TIME AND SETTING:
An in vitro contrast observation was performed in College of Marine Life Science, Ocean University of China from January to July 2008.MATERIALS:
Oral squamous carcinomas cells were sincerely presented by Professor Chen from the Ninth People's Hospital of Shanghai; ADM-HA-SSL (drug loading 156 mg/L) was sincerely presented by Professor Liu from College of Marine Life Science, Ocean University of China.METHODS:
Oral squamous carcinomas cells were cultured with 0.5, 1.0, 5.0, and 10.0 mg/L ADM-HA-SSL. MTT assay was used to detect the targeted cytotoxicity of ADM-HA-SSL against oral squamous cell carcinomas. With the concentrations of 5.0 and 10.0 mg/L, cell apoptosis was ascertained by cell flow cytometry after 6, 12, 24 and 48 hours.MAIN OUTCOME MEASURES:
Cell survival rate and apoptosis rate.RESULTS:
At 24 and 48 hours after induction, cytotoxicity assay revealed that the effect of ADM-HA-SSL was superior to that of free ADM (t=5.78−42.05, P < 0.01). The results of flow cytometry showed that the apoptosis rate was enhanced with the increase of the time (F=4 200.40, 4 775.36, P < 0.01), and the rate was also increased at the same time point with the increase of concentration (t=12.06-20.08, P < 0.05).CONCLUSION:
ADM-HA-SSL can specifically recognize oral squamous carcinomas cells and deliver adriamycin into the cells. And the effect is enhanced by the time prolonging.