Effects of Alendronate sodium on tumor necrosis factor alpha secreted from periprosthetic interface membranes of hip joints

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Abstract

BACKGROUND:

Studies regarding tumor necrosis factor α (TNF-α) is a newly entrance point in preventing and curing the difficult clinical problems of periprosthetic osteolysis. However, studies about the effect and mechanism of alendronate sodium on TNF-α are few.

OBJECTIVE:

To explore the effect of alendronate sodium on TNF-αsecretion of periprosthetic interface membranes of hip joints.

DESIGN, TIME AND SETTING:

A single sample contrast observation was performed at the Laboratory of Orthopedics in the Affiliated Orthopedic Hospital of Guangzhou University of Chinese Medicine.

MATERIALS AND PARTICIPANTS:

Periprosthetic interface membranes (15 g) were obtained from the First Affiliated Hospital of Guangzhou University of Chinese Medicine; 6 male New Zealand white rabbits were prepared for serum containing alendronate sodium. The alendronate sodium was manufactured by Shijiazhuang Pharmaceutical Factory.

METHODS:

Periprosthetic interface membranes (15 g) dissected aseptically from femurs were cultured in RPMI culture medium, periprosthetic interface membranes were sectioned into 30 fragments with weighing about 250 mg per fragment. The 30 fragments were randomly divided into control group, low concentration group (100 g/L) and high concentration group (200 g/L), with 10 samples in each group. Totally 1.8 mL, 1.6 mL and 1.8 mL culture medium were added into low concentration, high concentration and control groups respectively. Then 0.2 mL, 0.4 mL serum containing Alendronate was added respectively in low concentration, high concentration groups, 0.2 mL control serum was added in the control group. After addition of serum, the tissue suspension was incubated at 37 °C in saturation humidity with 5% CO2 for 72 hours.

MAIN OUTCOME MEASURES:

Expression of TNF-α in periprosthetic interface membranes of all groups were measured using ELISA methods.

RESULTS:

TNF-α secretion of periprosthetic interface membranes in low concentration, high concentration groups were (3.93±0.03), (3.92±0.04) pg/g, respectively, which had significant difference compared to the control group [(4.04±0.13) pg/g] (P < 0.01).

CONCLUSION:

Alendronate sodium significantly inhibits TNF-α secretion of periprosthetic interface membranes of hip joints.

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