SymbolChondrometaplasia of bone marrow mesenchymal stem cells of different resourcesin vivo

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Abstract

BACKGROUND:

Bone marrow mesenchymal stem cells (BMSCs) can repair cartilage defects after induction in vitro. However, most of the seed cells come from autos or allograft currently.

OBJECTIVE:

To observe the effects of the chondrometaplasia of homologous and heterogeneous BMSCs on repairing the laryngeal cartilage defects.

METHODS:

The third generation of human embryonic BMSCs and rabbit BMSCs were involved. The two kinds of cells grew on poly lactic acid-glycolic acid scaffold, and were inducted to cartilage cells after adding transforming growth factor β1 and cartilage morphogenetic proteins into the scaffold. The two kinds of cell systems were implanted in New Zealand rabbits. The defects were removed at 4 and 8 weeks after implantation for general and histological observation.

RESULTS AND CONCLUSION:

The human embryonic BMSCs and rabbit BMSCs were filled with regenerated tissues at 4 and 8 weeks after implantation. Histological observation showed that most cells in the tissues were cartilage cells which could secrete glycosaminoglycan and collagen 11. The two types of cell scaffold could generate cartilage cells in about the same amount, and there was no immunological rejection between them. Results revealed that the chondrometaplasia of the heterogeneous BMSCs induced by transforming growth factor β1 and cartilage morphogenetic proteins is similar to the homologous BMSCs for repair of the laryngeal cartilage defects.

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