SymbolDose-dependent effect of erythropoietin on the proliferation of rabbit bone marrow mesenchymal stem cells

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Some studies have certificated that erythropoietin (EPO) can promote bone marrow mesenchymal stem cells (BMSCs) proliferation, but it is not yet clear about the dose-dependent relationship.


To explore the different-dose effects of EPO on rabbit BMSCs proliferation activity and cell cycle.


MTT colorimetric test and flow cytometry were used to detect the effect of EPO (0, 2, 4, 8, 16 U/mL) on BMSCs cell cycle; meantime, the culture medium was collected for matrix metalloproteinase-2 (MMP-2) content detection.


2–16 U/mL EPO could promote BMSCs proliferation gradually in a dose-dependent manner. BMSCs percentage in S phase and proliferative index gradually increased with the dose increase of EPO, and the proliferation index of BMSCs with EPO was significantly higher than that of BMSCs without EPO (P < 0.05); however, the level of MMP-2 was decreased gradually, especially in 16 U/mL EPO group. These indicate that EPO can increase the proliferative ability of BMSCs in a dose-dependent manner.

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