Some studies have certificated that erythropoietin (EPO) can promote bone marrow mesenchymal stem cells (BMSCs) proliferation, but it is not yet clear about the dose-dependent relationship.OBJECTIVE:
To explore the different-dose effects of EPO on rabbit BMSCs proliferation activity and cell cycle.METHODS:
MTT colorimetric test and flow cytometry were used to detect the effect of EPO (0, 2, 4, 8, 16 U/mL) on BMSCs cell cycle; meantime, the culture medium was collected for matrix metalloproteinase-2 (MMP-2) content detection.RESULTS AND CONCLUSION:
2–16 U/mL EPO could promote BMSCs proliferation gradually in a dose-dependent manner. BMSCs percentage in S phase and proliferative index gradually increased with the dose increase of EPO, and the proliferation index of BMSCs with EPO was significantly higher than that of BMSCs without EPO (P < 0.05); however, the level of MMP-2 was decreased gradually, especially in 16 U/mL EPO group. These indicate that EPO can increase the proliferative ability of BMSCs in a dose-dependent manner.