SymbolBone marrow mesenchymal stem cells modified by glial cell line-derived neural factor gene co-cultrued with neurons subjected to anoxia-reoxygenation

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Abstract

BACKGROUND:

Preliminary studies in workgroup indicated that glial-derived neurotrophic factor (GDNF) gene modified by bone marrow mesenchymal stem cells (BMSCs) (BMSCs/GDNF) can stably express them RNA of GDNF, but whether GDNF protein in BMSCs/GDNF affects neurons subjected to anoxia-reoxygenation positively is unclear.

OBJECTIVE:

To observe the protective effects of BMSCs/GDNF on neurons subjected to anoxia-reoxygenation.

METHODS:

BMSCs/GDNF were co-cultured with anoxia-reoxygenation nerve cells. Hoechst33258 staining was used to detect apoptosis of neurons. Immunofluorescent-chemistry method was used to detect growth-associated protein-43 (GAP-43) expression in co-cultured cells.

RESULTS AND CONCLUSION:

The rate of neurons apoptosis in the BMSCs/GDNF group was lower than that of the BMSCs group and anoxia group (P < 0.05). Absorbance of growth-associated protein-43 (GAP-43) expression in the BMSCs/GDNF group was higher than that in the BMSCs group (P < 0.05). It is indicated that BMSCs/GDNF have neuroprotective effects due to inhibiting apoptosis of neurons subjected to anoxia-reoxygenation and increasing GAP-43 expression in co-cultured cells.

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