Studies have found that the cord blood contains a large amount of mesenchymal stem cells (MSCs). These cells have quicker speed of getting into cell cycle and stronger autocrine ability of growth factors than those in the bone marrow or peripheral blood after activation.OBJECTIVE:
To investigate the differentiation ability of human umbilical cord blood MSCs (HUMSCs) by serum of patients with severe liver disease.METHODS:
HUMSCs were isolated and purified by density gradient centrifugation and adherent culture. HUMSCs were induced to differentiate with 20% serum of liver failure patients. On days 7, 14, 21 after induction, the expressions of albumin and cytokeratin 18 were identified by immunohistochemical method.RESULTS AND CONCLUSION:
High purity HUMSCs were isolated from umbilical cord blood. HUMSCs strongly expressed CD44 and CD29, but did not express CD34. HUMSCs may differentiate into adipocytes in vitro. Morphological changes happened in HUMSCs cultured in L-DMEM medium containing 20% serum of patients with severe liver disease, the cells became larger and flat showing epithelioid cells type. Streptavidin-perosidase showed that only few cells were positive for albumin and cytokeratin 18 in the experimental group after 7 days induction. The positive rates of albumin and cytokeratin 18 were increased after 14 and 21 days induction, which were significantly different between the experimental group and control group (P < 0.05). It is indicated that high purity HUMSCs can be isolated by density gradient centrifugation and adherent culture, and serum of liver failure patients can induce the albumin and cytokeratin 18 expression in HUMSCs.