SymbolOptimizing transfection of human umbilical cord-derived mesenchymal stem cells using novel chemical transfection reagents

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Abstract

BACKGROUND:

Genetic modification technology of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) is numerous and varies in the complexity of their efficiency, reliability and safety.

OBJECTIVE:

To compare transfected efficiency of different novel chemical transfection reagents on hUCMSCs.

METHODS:

The hUCMSCs were transfected with Fugene HD, Lipofectamine LTX, and Attractene. Positive cells were detected under fluorescent microscope, and expression efficiencies were measured by using flow cytometry. Cell viability was measured by using trypan blue exclusion after transfection.

RESULTS AND CONCLUSION:

The highest transfected efficiency was observed with Lipofectamine LTX (32.5±2.12)% (P < 0.05), followed by Fugene HD (4.3±0.64)%, minimal transfection efficiency was observed with Attractene (1.74±0.08)%. Lipofectamine LTX demonstrated the lower cell viability (69.8±6.3)% compared with Fugene HD (92.4±4.2)% and Attractene (106.6±3.9)% (P > 0.05), but the difference was not significant. In summary, this study demonstrates that Lipofectamine LTX is a relative ideal chemical transfection reagent for hUCMSCs.

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