SymbolEffect of X box-binding protein 1 on the proliferation of endogenous neural stem cells after focal cerebral ischemia

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Abstract

BACKGROUND:

Nuclear transcription factor X box-binding protein 1 (Xbp1) is expressed during the development of the central nervous system, and plays an important role in neurons cell proliferation.

OBJECTIVE:

To investigate the expression of nuclear transcription factor Xbp1 in neural stem cells of cerebral ischemia/reperfusion hippocampal tissue and the inhibition effects of basic fibroblast growth factor (bFGF).

METHODS:

A novel model of cerebral ischemia/reperfusion was established with the method of middle cerebral artery occlusionin rats. The rats were divided into control group, ischemia/reperfusion group and bFGF group. The expression of BrdU and Xbp1 and the inhibition effects of bFGF in hippocampal neural stem cells were detected with immunohistochemical method.

RESULTS AND CONCLUSION:

After 3 days, BrdU positive cells in the hippocampus were obviously increased. To the 7th day, BrdU positive cells were more than those at any time. At 3 days, Xbp1 reaction products in ischemia/reperfusion were more than that in control group, and reached the peak on day 7, and then gradually decreased. bFGF can promotes the expression of Xbp1 in the hippocampus tissue and the proliferation of neural stem cells. These findings indicate that bFGF promotion of neural stem cells proliferation may be mediated by Xbp1 signaling pathway.

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