SymbolProliferation and directed differentiation of neural stem cells and olfactory ensheathing cells after co-transplantation into the brain of Alzheimer's disease rats

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Abstract

BACKGROUND:

The number of neurons is reduced in the brain of Alzheimer's disease rats, and the proliferation and differentiation ability of transplanted neural stem cells (NSCs) into neurons is limited.

OBJECTIVE:

To investigate the effects of olfactory ensheathing cells (OECs) on the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation into the brain of Alzheimer's disease rats.

METHODS:

OECs and NSCs were separately cultured in vitro, and the NSCs labeled with 5-bromodeoxyuridine (BrdU) before transplantation. Normal saline, NSCs and NSCs+OECs were separately transplanted into the hippocampi of Alzheimer's rats. At 7, 14, 21 and 28 days after transplantation, the immunohistochemical staining was used to detects the expression of BrdU and choline acetyltransferase (ChAT) in the slice of rat brain tissues to analyze the transplanted cells’ proliferation and differentiation.

RESULTS AND CONCLUSION:

The condition of proliferation and differentiation of the NSCs co-transplanted with OECs was the best. The BrdU-positive cells and ChAT-positive cells of the OECs and NSCs co-transplantation group were the most of the three groups (P < 0.01). OECs can promote the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation in the brain of Alzheimer's disease rats.

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