SymbolBone marrow mesenchymal stem cells improve cardiomyocyte contractility in rats with heart failure

    loading  Checking for direct PDF access through Ovid

Abstract

BACKGROUND:

Bone marrow mesenchymal stem cells (BMSCs) transplantation can significantly improve cardiac function.

OBJECTIVE:

To investigate the effect of BMSCs on cardiomyocytes contractility and dilatation in rats with heart failure after myocardial infarction.

METHODS:

Left anterior descending coronary arteries of 10 Sprague Dawley rats were ligated in order to establish the myocardial ischemia model. The model rats were randomly divided into two groups: the experimental group and the control group. Four weeks later, 5 × 106/L PKH26 labeled BMSCs were injected into the infracted myocardium in the experimental group and in the control group, only injected with PBS.

RESULTS AND CONCLUSION:

Compared with ligation, the cardiac function in two groups after modeling for 4 weeks was improved significantly (P < 0.01). After treated with BMSCs administration, the cardiac function in experimental group was improved significantly (P < 0.05). The amplitude of shortening/relengthening and contractile velocity of cardiomyocytes in experimental group was significantly increased (P < 0.01). The cardiomyocytes with contractility differentiated from BMSCs could be seen under fluorescence microscope. The injection of BMSCs could significantly increase the shortening/relengthening function of rat cardiomyocytes and was one of the important mechanisms in BMSCs improved of heart failure rats.

Related Topics

    loading  Loading Related Articles