Transplantation of endothelial progenitor cells provides us a new treatment options on limbs ischemia which is hard to cure in clinical.OBJECTIVE:
To estimate the effect of transplantation of endothelial progenitor cells (EPCs) derived from human peripheral blood on the improvement of limbs ischemia.METHODS:
The mononuclear cells derived from human peripheral blood were obtained by Ficoll density gradient centrifugation. The expressions of specific markers of EPCs were detected after cultured and amplified in vitro for 6 days, and the adherence cells marked by fluorochrome were multipoint injected into the nude mouse model with hind limb ischemia in order to evaluate the therapeutic effect.RESULTS AND CONCLUSION:
The adherence cells derived from mononuclear cells could express CD133, CD34 and vascular endothelial growth factor 2 (VEGFR-2). It indicated that we could culture and obtain the EPCs from mononuclear cells of human peripheral blood in vitro under certain condition. The necrosis and capillary density of the ischemia limbs of nude mice were improved obviously after EPCs transplantation (P < 0.05). Red and flavo-green fluorescently-labeled EPCs were observed in the paraffin section of ischemia limbs muscle. The transplanted EPCs can incorporate into the local capillary networks and improve the ischemia of posterior limb of nude mice.