SymbolEffect of recombinant retrovirus pLXSN/basic fibroblast growth factor transfection on osteogenesis of human marrow stroma cells

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Abstract

BACKGROUND:

In home and abroad have many studies on basic fibroblast growth factor (bFGF) transfection for improving the growth of muscle and vessel, but little studies on bFGF for improving the growth of osteogenesis.

OBJECTIVE:

To investigate the effect of recombinant retrovirus pLXSN/bFGF transfection on osteogenic potential of human marrow stroma cells (hMSCs).

METHODS:

hMSCs isolated from health volunteers, cultured and purified in vitro were randomly divided into four groups. Retrovirus pLXSN/bFGF group with recombinant retrovirus pLXSN/bFGF was added into culture medium. Retrovirus pLXSN group with recombinant retrovirus pLXSN was added to take the place of retrovirus pLXSN/bFGF. Positive control group with dexamethasone, glycerophosphate and ascorbic were added into culture medium. Blank control group without any special treatment.

RESULTS AND CONCLUSION:

hMSCs were uniform spindle-shape fibroblast after several passages. After treatment, hMSCs in retrovirus pLXSN/bFGF and positive control groups gradually showed flat shape. Immunohistochemical staining showed that the level of bFGF expression in retrovirus pLXSN/bFGF group was higher than that of the other three groups. Mineralized nodules and bone collagen were formed, and alkaline phosphatase activity was improved in retrovirus pLXSN/bFGF and positive control groups. Recombinant of retrovirus pLXSN/bFGF gene transfection into hMSCs can promote the osteogenic potential of hMSCs.

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