In recent years, studies have shown that toll-like receptors and nuclear factor κB (NF-κB) have a close relation with the occurrence and development of leukemia. However, there were no reports about the effect of scorpion venom on toll-like receptors on the surface of tumor cells and NF-κB.OBJECTIVE:
To investigate the effect of scopion venom component III (SVC-III) on expression of toll like receptor 4 (TLR4) and p65 in lipopolysaccharide (LPS) stimulated human leukemia monocytic THP-1 cells.METHODS:
THP1 cells were inoculated in 24-well cells culture plates, and there were five groups: control group, LPS (1 mg/L) stimulation group and SVC-III treatment group (LPS+ SVC-III, 1, 10, 20 mg/L). Real-time PCR was employed to detect the expression of TLR4 and p65 mRNA in THP1 cells, Western blot was used to detected the expression of TLR4 and p65 protein.RESULTS AND CONCLUSION:
The expression of TLR4 and p65 mRNA and protein of THP1 cells in LPS stimulation group was significantly higher than that in the control group (P < 0.01). However, the expression of TLR4 and p65 mRNA and protein of THP1 cells in SVC-III treatment group was lower than that in LPS stimulation group (P < 0.01) and in dose-dependent manner. SVC-III can down-regulate the expression of TLR4 and inhibit the activity of NF-κB in LPS stimulated THP1 cells in order to inhibit the cells proliferation.