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Abstract

BACKGROUND:

Smoking is an important risk factor for many vascular ischemic diseases, such as arterial occlusive and thromboangiitis obliterans. However, the exact mechanism behind this is unclear.

OBJECTIVE:

To investigate the effects of cigarette smoke extract on human umbilical vein endothelial cells and the intervention effect of atorvastatin during this process.

METHODS:

Human umbilical vein endothelial cells were cultured in vitro and divided into three groups, control group, cigarette smoke extract group and cigarette smoke extract plus atorvastatin group. Control group were cultured in DMEM medium containing 10% fetal bovine serum. Cigarette smoke extract group were cultured in DMEM medium containing 10% fetal bovine serum and 10% cigarette smoke extract. Cigarette smoke extract plus atorvastatin group were cultured in DMEM medium containing 10% fetal bovine serum, 10% cigarette smoke extract and 10 μmol/L atorvastatin.

RESULTS AND CONCLUSION:

Compared with control group, the cell morphology in cigarette smoke extract group was much irregular, the survival rate, the nitric oxide level in cell supernatant and the enzyme activity of nitric oxide synthase was decreased (P < 0.05). The cell morphology in cigarette smoke extract plus atorvastatin group was improved, the survival rate, the nitric oxide level in cell supernatant and the enzyme activity of nitric oxide synthase was increased (P < 0.05). It indicates that atorvastatin can protect vein endothelial cells from the damage effects of cigarette smoke extract. The mechanism may be related to the endothelium cell growth, the enzyme activity of nitric oxide synthase and the release of nitric oxide.

RESULTS AND CONCLUSION:

Huang X, Dai YB, Wu TF, Wang LW, Tang WX. Human umbilical vein endothelial cells damaged by cigarette smoke extract and intervention of atorvastatin. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(50): 9339-9343. [http://http://www.crter.cn http://en.zglckf.com]

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