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Abstract

BACKGROUND:

Establishment of a stable and effetive experimental animal model with congenital scoliosis is a precondition to lucubrate the congenital scoliosis with spinal cord injury and an efficient path to analyze the mechanism of spinal cord injury.

OBJECTIVE:

To assess the stability of congenital scoliosis model accompanied with complex spinal cord injury in rats.

METHODS:

A number of 15 model rats with no obvious spinal cord symptoms were put into special glass tubes with a diameter of 6 centimeters. The rats were kept in upright position in the tubes for 6 to 10 days to establish the congenital scoliosis model accompanied with complex spinal cord injury.

RESULTS AND CONCLUSION:

A total of 13 rats showed bilateral hind limb paralysis, adiaphoria of hind limbs and tail and no response to acupuncture and urination disorder. There were no obvious obstacles of defecation. The fur of bilateral hind limbs and tail in all the rats started to wither, became dull or even dropped. The mean Basso, Beattie and Bresnahen score was 7.3±2.2. Modified Tarlov scores were as follows, 0, 5 rats; 1, 2 rats; 2, 4 rats; 3, 1 rat; 4, 1 rat; 5, 0 rat. According to imaging, the congenital scoliosis were all over the spinal cord. The wedged hemivertebra and intercalated disc at the scoliosis vertex moved towards the spinal canal in some rats. According to histochemical results, there was a one-to-one relationship between the congenital defect with bone-and-spinal nourishing vessels damage and the compression in spinal cord segments caused by the bone structure damage in the corresponding segments. There were visible vascular distribution deletion regions at the malformation vertex. The vascular tissue of the venoux plexus underwent apoptosis at the scoliosis vertex. These findings indicate that the symptoms of complex spinal cord injury accompanied with the congenital scoliosis model are similar to the symptoms progress of congenital scoliosis patients. The repeat rate of the rat model is 87% (13/15).

RESULTS AND CONCLUSION:

Lu M, Fishchenko VY, Ma HS, Ren DY, Zheng R, Zhang J. Stability of a geneogenous scoliosis experimental model accompanied with spinal cord injury. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(50): 9365-9368. [http://http://www.crter.cn http://en.zglckf.com]

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