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Abstract

BACKGROUND:

Heat shock transcription factor 1 has heat shock response effect.

OBIECTIVE:

To construct a eukaryotic expression vector containing mouse heat shock transcription factor 1 (HSF1).

METHODS:

Amplified mouse HSF1 cDNA fragment was cloned into pcDNA3.1 to construct a eukaryotic expression vector pcDNA3.1-HSF1.

RESULTS AND CONCLUSION:

There were two fragments in electrophoresis after restriction enzyme digestion, 5.5 kb fragment and 1.5 kb fragment. The result of DNA sequencing showed that the sequence of the cloned HSF1 was identical to that GeneBank had reported. HSF1 protein band with the relative molecular mass of 72 000 was detected by western blot. These findings indicate that the eukaryotic expression vector pcDNA3.1-HSF1 containing mouse HSF1 is successfully constructed.

RESULTS AND CONCLUSION:

Ouyang HW, Tan J, Li GF, Luo CQ. Construction of a eukaryotic expression vector containing mouse heat shock transcription factor 1. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(50): 9425-9427. [http://http://www.crter.cn http://en.zglckf.com]

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