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Abstract

BACKGROUND:

Monocyte chemotactic factor can promote arterialization of newly formed vessels to form functional arteriole and then realize angiogenesis.

OBJECTIVE:

To construct Ad-EGFP-MCP-1 vector and to observe its expression in vivo after package, purification and viral titer detection.

METHODS:

MCP-1 gene was amplified by PCR and the sequence was compared with Genebank data, and then sub-cloned into the pDC315-EGFP vector after sequence analysis. Ad-EGFP-MCP-1 was obtained with pBHG lox ΔE1,3 Cre secondary packaging system. After transfection of Ad-EGFP-MCP-1 via trachea, EGFP expression was observed under fluorescence microscope.

RESULTS AND CONCLUSION:

The full-length of MCP-1 was obtained by PCR and identified by sequencing. Ad-EGFP-MCP-1 recombinant adenovirus vector was stably expressed in rat lung tissue. EGFP expression reached the peak level at 1 week and maintained this level for approximately 4 weeks. Ad-EGFP-MCP-1 recombinant adenovirus vector was successfully constructed, packaged and amplified, and it is effectively expressed in the lung tissue.

RESULTS AND CONCLUSION:

Zhang F, Wang W, Xie Y, Zhang H. Construction and expression of Ad-EGFP-MCP-1 vector. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(50): 9428-9432. [http://http://www.crter.cn http://en.zglckf.com]

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