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Abstract

BACKGROUND:

Carnitine palmitoyltransferase-II (CPT-II) is located in the inner mitochondrial membrane and is one of the pivotal enzymes during the fatty acid oxidation. Currently, there are no studies about influenza virus infection and variation of CPT-II gene.

OBJECTIVE:

To construct the plasmid expressing exon-4 of CPT-II gene (CPT-II-E4) and analyze its mutation.

METHODS:

Human CPT-II DNA from the blood of two patients infected with influenza virus was involved. After the PCR amplification, the product of CPT-II-E4 was cloned into pGEMD-T vector. The T-CPT-II E4 was transferred to E. coli DH5α cells after prepared with T4 DNA higase. Then propagation of bacteria, preparation of plasmid, EcoRI digestion, sequencing, and variation analysis were performed.

RESULTS AND CONCLUSION:

The recombinant pGEM-T-CPT-II E4 was proved to contain whole sequence of CPT-II-E4 with 1305 nucleotides which could code 435 amino acids. According to the original sequence from Genebank, two variable sites were found at 1618 (GTC→ATC) and 1858 (TTT→TCT) which code amino acids of V368I and F448L respectively.

RESULTS AND CONCLUSION:

Yao DB, Yao M, Wang L, Dong ZZ, Yao DF. Plasmid construction and variation analysis of exon-4 of carnitine palmitoyltransferase-II gene. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(50): 9437-9440. [http://http://www.crter.cn http://en.zglckf.com]

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