loading  Checking for direct PDF access through Ovid



The technique, which reinforces porous calcium phosphate scaffold by nano hydroxylapatite whisker in situ, can enhance the strength and toughness of scaffold.


To evaluate the biocompatibility of the nano-hydroxyapatite wisker and β-tricalcium phosphate (nHAw/β-TCP) as tissue engineered vascular scaffold in vitro.


The acute systemic toxicity test: Totally 30 mice were divided into intravenous injection group, intra-abdominal injection group and control group. Mice in the three groups were injected by leaching liquor and saline respectively, then the general state of the mice was recorded at the 24, 48 and 72 hours. Test of hemolysis: Dissolution of red blood cells were observed in the mixed liquids of leaching liquor and diluted blood, and the A value was detected at optical density 545 nm then the hemolytic rare was calculated. The sensitization test: Sixteen guinea pigs were randomly divided into experimental group, negative control group and postive control group. Leaching liquor from nHAw/β-TCP, normal saline and 2% dinitrofluorobenzene were injected intradermally into each side of the backbone of each guinea pig. The erythema and edema were recorded at immediate, 24, 48, 72 hours. Test of cytotoxicity: The cells were cultured in the special L-DMEM that nHAW/β-TCP were dipped in and then general morphology of cells and cell activity were observed through microscope and CCK-8.


The acute systemic toxicity test: After intravenous and intra-abdominal injection of soaked of materal, there were no changes in breath, appetite, body weight of mice and no death. Test of hemolysis: The haemolytic rates of nHAw/β-TCP were less than 5% of international standard organization requirements, suggesting these materaials had no haematolysis effect. The sensitization test: No allergic reaction were found in guinea pig after endodermal injection. Test of cytotoxicity: Test of CCK-8 colormetry showed that the different densities leaching liquor had no cytotoxicity. nHAw/β-TCP does not cause acute whole body toxic reaction, haematolysis effect, allergic reaction and no cytotoxity. The materials have a good biocompatibility and meet the application requirements for tissue engineered vascular scaffold.


Hu HT, Xu GH, Liu JC, Jiang YQ, Tang DZ, Zan QF, Ye XJ. Biological security of nano-hydroxyapatite wisker and β-tricalcium phosphate composited bone substitute. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(51): 9575-9578. [http://www.crter.cn]

Related Topics

    loading  Loading Related Articles