Biological effects of various pure titanium implant surface micro-arc oxidation coatings are different.OBJECTIVE:
To study the effects of three different micro-arc oxidation coating palatal implant titanium tablets on mouse osteoblast cell proliferation, alkaline phosphatase activity and beta 1-integrin gene expression level.METHODS:
Using international common mice lines (MC3T3-E1), three different coated titanium pieces were used as influencing factors, pure titanium group as control group. MTT method and electron microscopic observation were conducted for cell adhesion and cell proliferation, PNPP was used for determination of alkaline phosphatase activity, RT-PCR method to detect beta 1-integrin expression in mouse osteoblasts.RESULTS AND CONCLUSION:
The MTT value, alkaline phosphatase activity, beta 1-integrin gene expression level and electron microscopic observation showed calcium, phosphorus, magnesium, zinc coated titanium pieces of titanium dioxide had the best biocompatibility, titanium dioxide coating titanium containing calcium containing phosphorus salt was secondary, and titanium dioxide coated titanium slice was the worst. The results showed that mouse osteoblasts on the porous of calcium, phosphorus, magnesium, zinc coated titanium pieces had the best adhesion and proliferative abilities.