SymbolSecurity for adenovirus-mediatedp53 gene transfer to the donor heart

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Abstract

BACKGROUND:

Wild-type p53 gene transfer to the donor heart can greatly inhibit graft coronary artery intima hyperplasia and lumen narrowness.

OBJECTIVE:

To study the security of adenoviral-mediated wild-type p53 gene transfer to the donor heart after heart transplantation.

METHODS:

Rat models of heterotopic (abdomen) heart transplantation were developed. Wistar rats served as donors and SD rats as recipients. After donor hearts were removed, 800 μL adenoviral vector encoding the wild-type p53 gene (Ad-p53 group), adenoviral vector encoding the β-galactosidase gene (LacZ) (Ad-LacZ group) or saline (control group) were infused into the donor heart respectively before transplantation. The donor heart was stored in the 4° saline for 30 minutes before heart transplantation. At 5 days after operation, P53 protein expressions in coronary artery of donor hearts were tested by western blot analysis. At 28 days after transplantation, the serum specimen was collected for the biochemical indicators, and the major organs of the recipients were tested by the histopathological analysis and the reverse transcription polymerase chain reaction of the adenoviral E1A sequences.

RESULTS AND CONCLUSION:

The expression of P53 protein was found in donor hearts in Ad-p53 group at 5 days after operation, and no expression in Ad-LacZ group and control group. At 28 days after operation, rat serum biochemistry values in three groups was normal, the major organs of the recipients were not affected seriously, no virus spread to other organs in this experimental protocol. The results confirmed that the ex vivo adenoviral-mediated gene transfer to the donor heart via the coronary artery during the heart transplantation is safe.

RESULTS AND CONCLUSION:

Wang LP, Song FF, Li XL, Liu Y, Jia ZB, Yin XH. Security for adenovirus-mediated p53 gene transfer to the donor heart. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(53): 9961-9964. [http://www.crter.cnhttp://en.zglckf.com]

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