SymbolEffects of species, development periods and different experimental conditions on the outcomes of embryo freezing and thawing in cleavage stage

    loading  Checking for direct PDF access through Ovid

Abstract

BACKGROUND:

In the studies of frozen-thawed human cleavage embryos, whether the experimental conditions and experimental animal model have the same sensitivity with human to reflect the experiment quality is worth exploring. OBJECTIVE: To investigate the effects of embryo species, development periods and different frozen conditions on the outcomes of embryo freezing and thawing in cleavage stage.

METHODS:

Human embryos in cleavage stage served as control group, KM mouse embryos were randomly divided into 2-cell-stage group, 4-cell-stage group and 8-cell-stage group. Experiment scheme: Operating environment temperature were 18-20°, 24-26° and 37°. Slow programmed cryopreservation, self-made Straw-leaf vitrification cryopreservation and close pulled straw vitrification cryopreservation were used in this study. Contact durations of vitrified solution were less than 40 seconds, 40-60 seconds and 60-90 seconds. The embryo survival rates and developmental rates after cultivating for 24 hours were compared among groups.

RESULTS AND CONCLUSION:

The survival rate of control group under the environment temperature of 24-26° was higher than that of 37° temperature (P < 0.05). The survival rate of control group and 4-cell-stage group by self-made Straw-leaf vitrification cryopreservation was higher than that of the two groups by slow programmed cryopreservation (P < 0.05). The embryo developmental rates after cultivating for 24 hours showed no significant difference (P > 0.05). There was no significant difference in embryo survival rates among groups with different contact durations of vitrified solution (P > 0.05). These findings suggest that the cleavage embryo resuscitation effect of vitrification is better than that of slow recovery process. Under the temperature of 24-26 ?, the outcomes of embryo freezing and thawing can be improved by reducing cryoprotectant dose and contact duration. Different embryo species and developmental stages may produce different outcomes under the same conditions. Compared with other stages of embryo development, 4-cell mouse embryo is a more suitable experimental model for the freezing and thawing studies of human cleavage stage embryos.

RESULTS AND CONCLUSION:

Gong XY, Zhao J, Hu B, Wang P, Cai X. Effects of species, development periods and different experimental conditions on the outcomes of embryo freezing and thawing in cleavage stage. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011; 15(53): 9973-9976. [http://www.crter.cnhttp://en.zglckf.com]

Related Topics

    loading  Loading Related Articles