Symbol

    loading  Checking for direct PDF access through Ovid

Abstract

BACKGROUND:

Animal experiments and preliminary clinical studies have demonstrated that transplantation of stem cells can replace necrotic myocardial cells, increase the number of myocardial cells with functions and improve cardiac function, thereby providing a brand-new pathway for treatment of myocardial infarction.

OBJECTIVE:

To investigate the effects of transplantation of autologous bone marrow mesenchymal stem cells and bone marrow mobilization after myocardial infarction on cardiac functions.

METHODS:

30 mL bone marrow was taken from pig anterior superior iliac spine for harvesting bone marrow mesenchymal stem cells. Fifteen pigs were assigned to three groups. In the model group, only myocardial infarction was induced. In the stem cell transplantation group, at 3 hours after myocardial infarction induction, bone marrow mesenchymal stem cells were transfused via coronary vein. In the stem cell mobilization group, at 3 hours after myocardial infarction induction, granulocyte colony-stimulating factor (150 μg/kg per day) was administered for a total of 5 successive days.

RESULTS AND CONCLUSION:

In the stem cell mobilization group and stem cell transplantation group, left ventricular end systolic diameter and left ventricular end-diastolic diameter were slightly, but not significantly, decreased, and ejection fraction was slightly, but not significantly, increased (P > 0.05) at 8 weeks after myocardial infarction compared with before infarction. In the stem cell mobilization group and stem cell transplantation group, vascular endothelial growth factor level was significantly increased at 8 weeks after myocardial infarction than before infarction (P < 0.05). At 8 weeks after myocardial infarction, capillary density at the infarcted zone was significantly greater in the stem cell mobilization group and stem cell transplantation group than in the model group (P < 0.05). These findings suggest that after myocardial infarction, autologous bone marrow mesenchymal stem cell transplantation and bone marrow mobilization can significantly improve cardiac function, but the precise efficacy requires further investigation via large-size sample.

Related Topics

    loading  Loading Related Articles