The future of cell therapy requires high purity of mesenchymal stem cells (MSCs) in order to clearly determine the therapeutic dose and dose-response relationship. Establishing a robust amplification system in vitro to obtain the therapeutic dose of stem cells while preserving the characteristics of stem cells is the urgent of clinical laboratories.OBJECTIVE:
To simulate a microenvironment for stem cells in vitro to harvest and expand umbilical cord blood-derived and cord-derived MSCs and to compare with conventional two-dimensional culture system.METHODS:
The umbilical cord blood-derived and cord-derived MSCs were inoculated in the conventional two-dimensional plastic culture system and extracellular matrix, respectively. The two amplification systems for in vitro large-scale expansion of umbilical cord blood-derived and cord-derived MSCs were evaluated from the points of cell counts and surface markers.RESULTS AND CONCLUSION:
The production of umbilical cord blood-derived and cord-derived MSCs in the bone marrow-derived extracellular matrix culture system was 4-6 times of that in the conventional two-dimensional culture system. FACS analysis showed that extracellular matrix system better maintained the surface marker of stem cells. Therefore, such a three-dimensional culture system is much closer to physical environment than the two-dimensional culture system. The established bone marrow-derived extracellular matrix culture system can maintain the characteristics of mesenchymal stem cells, which provides access to more homogeneous MSCs of high activity within a short time period.