Human umbilical cord mesenchymal stem cells (hUCMSCs) can regulate cellular biological activities by paracrine mechanism.OBJECTIVE:
To investigate the effects of hUCMSCs on the proliferation and apoptosis of endometrial cells.METHODS:
Endometrial cells and hUCMSCs were cultured in vitro. hUCMSCs and endometrial cells were co-cultured on the Transwell co-culture plate at a proportion of 1×105/1×105 to establish upper and lower double-cell non-contact co-culture system, serving as the experimental group. Endometrial cells (1×105 cells/well) were separately cultured as control group.RESULTS AND CONCLUSION:
MTT assay results showed that cell proliferation in the experimental group was significantly inhibited in a time-dependent manner compared with the control group (P < 0.05). Flow cytometry results showed that apoptotic cells were significantly more in the experimental group than in the control group (P < 0.05), and endometrial cells entering into S stage from G1 stage were reduced. RT-PCR results showed that PTEN mRNA expression was significantly higher in the experimental group than in the control group. These findings suggest that hUCMSCs inhibit the in vitro proliferation of human endometrial cells and promote their apoptosis by paracrine mechanism, which occurs possibly by upregulation of PTEN mRNA expression.