Human umbilical cord stromal stem cells are expected to become a new seed cell in the treatment of live cirrhosis. However, few studies of in vitro experiment have been reported.OBJECTIVE:
To explore the effect of human umbilical cord stromal stem cells-conditioned medium (USSC-CM) on the biological activity of fibrosis in rat hepatic stellate cells (HSCs), and the acting target.METHODS:
HSCs were seeded in the six orifice plate in proportion (1.0×105/mL), then cultured without serum for 24 hours; 2 mL USSC-CM were added to treat HSCs as experimental group; while HSCs were treated with equivalent fresh medium as control group. The expression of transforming growth factor β1 (TGF-β1), collagen-α1 and TIMP-2 mRNA in HSCs were detected by RT-PCR. The expression of TGF-β1 protein was determined by Western blot.RESULTS AND CONCLUTION:
The expression of TGF-β1, collagen-α1 and TIMP-2 mRNA in HSCs were down-regulated in experimental group compared to control group, and the tendency enhanced with time (USSC-CM treated 72 h > USSC-CM treated 48 h > control group). And the expression of TGFβ1 protein in HSCs were down-regulated in experimental group compared to control group and showed time dependent manner (control group > USSC-CM treated 48 h group > USSC-CM treated 72 h group, P < 0.05). Human umbilical cord stromal stem cells can inhibit biological activity of HSCs. And it may carry its point by focusing on the acting target related to TGF-β together with downstream production.