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Abstract

BACKGROUND:

At present, seed sources of stem cells engineering are diverse, and mesenchymal stem cells can be isolated from exfoliated cells in fetal amniotic fluid.

OBJECTIVE:

To investigate the effects of the media with different elements on human amniotic fluid-derived mesenchymal stem cells (h-AFMSCs) cultured in vitro, and to analyze the biological characterization of h-AFMSCs at optimum medium cultured in vitro in order to optimize the culture strategy of h-AFMSCs.

METHODS:

Amniotic fluid was extracted from pregnant women during 16 to 24 weeks of gestation, and then was used for primary culture and subculture. h-AFMSCs were cultured in media containing six different elements.

RESULTS AND CONCLUSION:

h-AFMSCs were isolated from amniotic fluid in mid-trimester. The proliferative ability of h-AFMSCs was improved to a maximal extent in the mixed medium of low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and MESEN PRO medium at a mixed ratio of 1:1. h-AFMSCs highly expressed CD29, CD73, CD90, CD105, CD166 and lowly expressed CD14, CD34, CD45, HLA-DR. Stem cells gene OCT-4 and Naong highly expressed in h-AFMSCs cultured in vitro. h-AFMSCs showed a higher osteogenic and adipogenic differentiation potential, and they could inhibit the proliferation of lymphocytes in vitro. It is indicated that mid-trimester amniotic fluid is a good cell source of h-AFMSCs with lower immunogenicity.

RESULTS AND CONCLUSION:

Wang PP, Zheng YB, Peng L, Yu J, Gao ZL. Biological characteristics and optimal strategy of human amniotic fluid-derived mesenchymal stem cells cultured in vitro.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1780-1785. [http://www.crter.cnhttp://en.zglckf.com]

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