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At present, seed sources of stem cells engineering are diverse, and mesenchymal stem cells can be isolated from exfoliated cells in fetal amniotic fluid.


To investigate the effects of the media with different elements on human amniotic fluid-derived mesenchymal stem cells (h-AFMSCs) cultured in vitro, and to analyze the biological characterization of h-AFMSCs at optimum medium cultured in vitro in order to optimize the culture strategy of h-AFMSCs.


Amniotic fluid was extracted from pregnant women during 16 to 24 weeks of gestation, and then was used for primary culture and subculture. h-AFMSCs were cultured in media containing six different elements.


h-AFMSCs were isolated from amniotic fluid in mid-trimester. The proliferative ability of h-AFMSCs was improved to a maximal extent in the mixed medium of low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and MESEN PRO medium at a mixed ratio of 1:1. h-AFMSCs highly expressed CD29, CD73, CD90, CD105, CD166 and lowly expressed CD14, CD34, CD45, HLA-DR. Stem cells gene OCT-4 and Naong highly expressed in h-AFMSCs cultured in vitro. h-AFMSCs showed a higher osteogenic and adipogenic differentiation potential, and they could inhibit the proliferation of lymphocytes in vitro. It is indicated that mid-trimester amniotic fluid is a good cell source of h-AFMSCs with lower immunogenicity.


Wang PP, Zheng YB, Peng L, Yu J, Gao ZL. Biological characteristics and optimal strategy of human amniotic fluid-derived mesenchymal stem cells cultured in vitro.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1780-1785. [http://www.crter.cn]

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