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Abstract

BACKGROUND:

Dlx2 plays an important role in the regulation of cranial neural crest cells migration and craniofacial skeleton development. However, the effects of Dlx2 on apoptosis and cell cycle in osteoblast differentiation are not reported.

OBJECTIVE:

To investigate the effects of Dlx2 overexpression on cell cycle regulation and apoptosis in MC3T3-E1 preosteoblast cells differentiation.

METHODS:

Anti-retroviral pMSCV-puro-Dlx2 was transfected into MC3T3-E1 cells which were cultured in mineralization induced fluid. MC3T3-E1-Dlx2 cell lines stably overexpressing Dlx2 were constructed. Cell lines of Dlx2 over-expression were determined by RT-PCR and western blot. Apoptosis was detected by flow cytometry after Annexin V/PI and PI/RNase staining and cell cycle changes were detected by flow cytometry after PI/RNase staining.

RESULTS AND CONCLUSION:

MC3T3-E1-Dlx2 cell lines was successfully constructed. Cell cycle was blocked in G1/G0 phase (P < 0.05) and apoptosis was up-regulated (P < 0.05) in Dlx2 overexpression cell model. Dlx2 overexpression reduces cell proliferation and promotes cell differentiation to exercise functions.

RESULTS AND CONCLUSION:

Sun H, Wang XD, Dai JW, Lu JT, Shen GF. Effects of Dlx2 overexpression on cell cycle regulation and apoptosis of preosteoblast cells MC3T3-E1 during osteogenic differentiation process.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1808-1812. [http://www.crter.cnhttp://en.zglckf.com]

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