Symbol

    loading  Checking for direct PDF access through Ovid

Abstract

BACKGROUND:

The model of sciatic nerve injury can be used to test the hyperalgesia and paraesthesia caused by nocuous heat stimulation and mechanical stimulation.

OBJECTIVE:

To investigate the expression of brain-derived neurotrophic factor (BDNF) in the spinal dorsal horn and dorsal root ganglion in a rat model of sciatic nerve injury after intrathecal transplantation of neural stem cells.

METHODS:

Seventy-two adult Sprague-Dawley rats were randomly divided into sham-operation, control and experimental groups. Rat model of sciatic nerve injury was established in the control and experimental groups. In the sham-operation group, only sciatic nerve was exposed, without ligation. Intratheal transplantation was performed at 3 and 10 days after induction of sciatic nerve injury. 30 μL of neural stem cell suspension was injected into the experimental group, and 30 μL cell culture solution was injected into the sham-operation and control groups.

RESULTS AND CONCLUSION:

Compared with sham-operation group, mechanical withdrawal threshold and thermal withdrawal latency were gradually reduced at 3 days after transplantation, decreased to the lowest level at 7 days (P < 0.01) and recovered to pre-transplantation level at 21 days in the control and experimental groups. At 7 and 14 days after transplantation, mechanical withdrawal threshold and thermal withdrawal latency were significantly increased in the experimental group than in the control group (P < 0.01). Compared with control group, at 7, 14 and 21 days after transplantation, BDNF expression was significantly decreased in the experimental group (P < 0.05). At 14 and 21 days after transplantation, BDNF expression was significantly higher in the experimental group than in the control group (P < 0.05). These findings show that intrathecal transplantation of neural stem cells can prevent and treat the neuropathic pain from peripheral nerve injury by increasing BDNF expression in the spinal dorsal horn and dorsal root ganglion.

RESULTS AND CONCLUSION:

Zhang MH, Hu YM, Zhang H, Hu YP, Liu QZ, Wang HC, Li WY. Brain-derived neurotrophic factor expression in the spinal dorsal horn and dorsal root ganglion in a rat model of sciatic nerve injury after intrathecal transplantation of neural stem cells.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1851-1855. [http://www.crter.cnhttp://en.zglckf.com]

Related Topics

    loading  Loading Related Articles