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Abstract

BACKGROUND:

Previous studies of microRNA-155 expression and function in macrophage predominantly focus on total mononuclear macrophage.

OBJCTIVE:

To evaluate the expression difference of microRNA-155 in M1 and M2 macrophages.

METHODS:

Bone marrow-derived macrophages were induced to differentiate towards M1 and M2 macrophages using interferon-γ (100 U/mL) + lipopolysaccharide (5 ng/mL) and interleukin-4 (10 ng/mL), respectively.

RESULTS AND CONCLUSION:

The differentiation proportion of M1 and M2 macrophages detected by flow cytometry was 91% and 95%, respectively. Reverse transcription PCR method showed that inducible nitric oxide synthase (iNOS) mRNA was highly expressed in M1 macrophages, but it was hardly expressed in M2 macrophages. Arginase-1 and found in inflammatory zone 1 mRNA expression was highly expressed in M2 macrophages, but the expression was low in M1 macrophages. Tumor necrosis factor α mRNA expression was significantly higher in M1 macrophages than in M2 macrophages, on the contrary, interleukin 10 mRNA expression was significantly higher in M2 macrophages than in M1 macrophages (P < 0.05). Real-time fluorescence quantitative PCR showed that microRNA-155 expression in M1 macrophages was significantly higher compared with that in M2 macrophages (P < 0.05). These findings suggest that MiRNA-155 can serve as a useful maker for differential diagnosis of M1 and M2 macrophages.

RESULTS AND CONCLUSION:

Liang YB, Wang ZH, Tang H, Ma ZF. Diverse expression patterns of microRNA-155 between bone marrow-derived M1 and M2 macrophages.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1866-1870. [http://www.crter.cnhttp://en.zglckf.com]

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