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Abstract

BACKGROUND:

Shear stress is an important non-pharmacological method to regulated endothelial progenitor cells (EPCs). However, the effect of shear stress on the anti-oxidative activity of EPCs is not clear.

OBJECTIVE:

To observe the effect of shear stress on the copper-zinc superoxide dismutase (Cu/Zn-SOD) gene expression and activity in EPCs, in order to investigate the regulatory effects of shear stress on the EPCs function.

METHODS:

The peripheral blood mononuclear cells of healthy adult were inducted into EPCs. Then, they were divided into four different experimental groups which included stationary group, low-flow shear stress group (0.05 mN/cm2), medium-flow shear stress group (0.15 mN/cm2) and high-flow shear stress group (0.25 mN/cm2).

RESULTS AND CONCLUSION:

The peripheral blood mononuclear cells were differentiated into EPCs. They presented typical “spindle-shaped” appearance, and were positively labeled by acetylated-LDL, lectin, FLK-1 and VWF. The Cu/Zn-SOD activity of human EPCs in shear stress treatment groups was higher than that in stationary group. The higher the shear stress, the higher the Cu/Zn-SOD activity of human EPCs. Quantitative real-time PCR indicated that shear stress could increase the CuZn-SOD mRNA expression of EPCs, the higher the shear stress, the stronger the CuZn-SOD mRNA expression of EPCs. In vitro shear stress could upregulate the gene expression of Cu/Zn-SOD in EPCs, increase the Cu/Zn-SOD activity in human EPCs, which contributes to enhance the anti-oxidative activity of EPCs. Therefore, enhanced shear stress within the physiological range could regulate the function of EPCs, which can act as an important strategy of enhancing their therapeutic approach for vascular endothelial injury.

RESULTS AND CONCLUSION:

Yang Z, Lai GH, Xia WH, Luo CF, Wang JM, Chen L, Liao XX, Jin YF, Tao J. Effect of shear stress on the copper-zinc superoxide dismutase gene expression and activity in human endothelial progenitor cells.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10): 1871-1874. [http://www.crter.cnhttp://en.zglckf.com]

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