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Heart development results from the interaction among various factors. Research on the interaction of Nkx2.5 and GATA4 may help us to further understand the process of heart development.


To research the interaction of cardiac transcription factor Nkx2.5 and GATA4 in vitro.


On the basis of the construction of plasmids of pCMV-Myc-GATA4, pCMV-HA-Nkx2.5 and pTal-luc-BNP, plasmids were transfected into COS7 cells. The interaction of Nkx2.5 and GATA4 was identified by co-immunoprecipitation and luciferase analysis. The possible synergistic activation of Nkx2.5 and GATA4 on BNP promoter sequence was analyzed by luciferase.


Co-immunoprecipitation experiments showed that Nkx2.5 and GATA4 could be precipitated by each other, demonstrating that Nkx2.5 and GATA4 could directly interact with each other in COS7 cells. The pTal-luc-BNP combined with Nkx2.5 and GATA4 were co-transfected into COS7 cells. Compared with single transfected with Nkx2.5 or GATA4 plasmid, co-transfected with Nkx2.5 and GATA4 displayed synergistic transcriptional activation of the BNP promoter. Nkx2.5 and GATA4 play important roles separately in the process of heart development, and the interactions of Nkx2.5 and GATA4 are also vital in normal heart development.


Ding JD, Fang X, Li KR, Yao YY, Tao SY, Wang D, Ma GS. Interaction of cardiac transcription factor Nkx2.5 and GATA4. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 1933-1936. [http://www.crter.cn]

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