There are individual differences in the patients suffered tumor. Therefore the research on the cancer line can not show accurate differences between individuals, while primary cell culture can show more similar characteristics to in vivo state.OBJECTIVE:
To establish a method in vitro for primary culture of cervical carcinoma cells.METHODS:
Cervical cancer cells were obtained from 23 fresh cervical cancer tissues using the trypsin and collagenase type I digestion, and their growth curve was detected. Cell surface markers of CK17and CD44 were identified with immunofluorescence staining.RESULTS AND CONCLUSION:
Cervical cancer cells were obtained from 21 of 23 cases, and the cells had the continuous passage and steady proliferation. Immunofluorescence staining showed that the cells positively expressed CK17 and CD44. It is indicated that primary cervical carcinoma cells can be obtained by trypsin and collagenase type I digestion.RESULTS AND CONCLUSION:
Ma XP, Cheng JX, Zhang Y, Yuan M. Culture in vitro and identification of human cervical carcinoma cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 1959-1962.