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Because of the damage of formalin-fixed paraffin-embedded tissue to DNA in the process of its production and preservation, it is difficult to extract high quality and sufficient DNA for PCR.


To analyze the influencing factors of the DNA extracted from paraffin-embedded tissue for PCR.


Genomic DNA was extracted from paraffin-embedded esophageal carcinoma tissue sections of 10.0 μm×2, 10.0 μm×4 and 10.0 μm×5. Template DNA amount of 0.05, 0.1, 0.2 μg was used to amplify gene β-actin, respectively. PCR cycle times were set as 35, 40 and 45. The influencing factors of PCR were analyzed.


The analysis of agarose gel electrophoresis showed that when paraffin-embedded esophageal carcinoma tissue section amount was 10.0 μm×2, the PCR cycle times were 40; when the template DNA amount was 0.05μg, the obtained target DNA was of the highest quality. It shows that reducing the amount of paraffin-embedded tissue and DNA template can be help to require high quality PCR strips; PCR cycle times should be more than 40 and less than 45, and if it increases beyond this range there is meaningless.


Ma LL, Ailijiang•Tuerxun, Zhang L. PCR amplication of the DNA extracted from paraffin-embedded tissues in different conditions. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 1973-1976. [http://www.crter.cn]

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