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Abstract

BACKGROUND:

Because of the damage of formalin-fixed paraffin-embedded tissue to DNA in the process of its production and preservation, it is difficult to extract high quality and sufficient DNA for PCR.

OBJECTIVE:

To analyze the influencing factors of the DNA extracted from paraffin-embedded tissue for PCR.

METHODS:

Genomic DNA was extracted from paraffin-embedded esophageal carcinoma tissue sections of 10.0 μm×2, 10.0 μm×4 and 10.0 μm×5. Template DNA amount of 0.05, 0.1, 0.2 μg was used to amplify gene β-actin, respectively. PCR cycle times were set as 35, 40 and 45. The influencing factors of PCR were analyzed.

RESULTS AND CONCLUSION:

The analysis of agarose gel electrophoresis showed that when paraffin-embedded esophageal carcinoma tissue section amount was 10.0 μm×2, the PCR cycle times were 40; when the template DNA amount was 0.05μg, the obtained target DNA was of the highest quality. It shows that reducing the amount of paraffin-embedded tissue and DNA template can be help to require high quality PCR strips; PCR cycle times should be more than 40 and less than 45, and if it increases beyond this range there is meaningless.

RESULTS AND CONCLUSION:

Ma LL, Ailijiang•Tuerxun, Zhang L. PCR amplication of the DNA extracted from paraffin-embedded tissues in different conditions. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 1973-1976. [http://www.crter.cnhttp://en.zglckf.com]

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