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Abstract

BACKGROUND:

Several studies have showed that there are different qualities of bacterial genome DNA extracted from the human intestine using different kits. Therefore, it is essential and urgent to select an effective, simple, and excellent DNA stool mini kit.

OBJECTIVE:

To compare the quality differences of bacterial genome DNA extracted from the human intestinal by using QIAamp and Biomed DNA Stool Mini Kits.

METHODS:

Thirty fresh fecal samples of healthy adult people were selected, and bacterial genome DNA was extracted using these two kits. Concentration, absorbance radio of 260 to 280 nm and extraction rate were measured. Species-specific primers for Lactobacillus group were designed by a set of 16S rDNA-targeted to conduct the general PCR using the genome DNA as the template. The electrophoresis strip’s number, brightness and density were compared after gel electrophoresis; subsequently, the number of Lactobacillus group was detected by fluorescent real-time PCR.

RESULTS AND CONCLUSION:

Concentration, extraction rate, expression level, Lactobacillus amount of bacterial genome DNA extracted from the human intestinal using QIAamp kit were higher than those using Biomed kit (P < 0.05 or P < 0.01). It suggests that the quality of genome DNA extracted with QIAamp kit is more excellent than that with Biomed kit.

RESULTS AND CONCLUSION:

Song MR, Yao P, Zhang YX. Quality differences of bacterial genome DNA extracted from the human intestine by using QIAamp and Biomed DNA Stool Mini Kits. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 2015-2018. [http://www.crter.cnhttp://en.zglckf.com]

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