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In recent years, there are a lot of researches on tissue culture and cell culture using chicken embryo. But neurocyte culture using chicken embryo has not been reported.


To summarize the research progress of tissue culture and cell culture using chicken embryo in order to find a convenient and inexpensive method of neurocyte culture.


An online search of CNKI and PubMed databases was performed for articles related to chicken embryo culture model and neurocyte culture published between January 1998 and June 2011, using key words of “chicken embryo chorioallantoic membrane model; neuronal culture” in Chinese and English. Articles related to chicken embryo culture model were selected. As to the in identical field, articles published recently or in authoritative magazine were of priority. A total of 205 articles were collected in the initial search, 34 of them were retained according to the inclusion criteria.


Chicken embryo is widely used in virus isolation, virus cultivation, vaccine preparation, mechanisms of tumor biology and drug screening. Compared with other culture methods, the chicken embryo culture model is cheap, convenient and rapid. Fully understanding and correctly utilization of the chicken embryo culture model provide scientific and feasible methods and train of thought for the research in many fields.


Wang Y, Li L, Li T, Duan YG. Application of chicken embryo in biomedical research.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(11): 2067-2070. [http://www.crter.cn]

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