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Abstract

BACKGROUND:

Polylactic acid is a widely used scaffold material, but its hydrophobicity and lack of cell recognition signal limit its application.

OBJECTIVE:

To investigate the feasibility of constructing a tissue engineered ureter using a modified polylactic acid/type I collagen compound scaffold in vitro.

METHODS:

Electrospinning polylactic acid naofibrous scaffolds were dipped into a acetic acid solution of type- I collagen to make the collagen absorbed onto the nanofiber surface. Then ureter epithelial cells were seeded separately onto the modified electrospun polylactic acid naofibrous scaffolds or control scaffolds.

RESULTS AND CONCLUSION:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results confirmed that there was higher cellular activity in the collagen modified compound scaffolds, which was superior to cells untreated electrospinning polylactic acid scaffold at each time point. Scanning electron microscope showed that the most part of the surface of the modified scaffold was covered with ureter epithelial cells at 5 days after seeding. The modified polylactic acid/type collagen compound scaffold can obviously increase adhesion, growth and proliferation of the seeded cells, and has potential applications in ureteral tissue engineering reconstruction.

RESULTS AND CONCLUSION:

Xu YD, Fu WJ, Shi JG, Tan HS, Zhang X. A modified electrospun polylactic acid/collagen scaffold for ureteral tissue engineering reconstruction in vitro. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2095-2098. [http://www.crter.cnhttp://en.zglckf.com]

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