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Abstract

BACKGROUND:

As non-viral gene vectors, chitosan have the low cytotoxicity, low immunogenicity, good biocompatibility, and high positive charge density. It can easily interact with negatively charged DNA through electrostatic interactions to form chitosan-DNA nanoparticles which can prevent nuclease degradation.

OBJECTIVE:

To prepare chitosan nanoparticles carrying recombinant human transforming growth factor β1 (TGF-β1) gene and to detect the sustained release efficiency and gene transfect against chondrocytes in vitro.

METHODS:

The chitosan/plasmid enhanced green fluorescent protein-TGF-β1 (chitosan/pEGFP-TGF-β1) nanoparticles were prepared by a complex coacervation method with chitosan and plasmid DNA (pDNA) which loaded EGFP gene and recombinant human TGF-β1 gene.

RESULTS AND CONCLUSION:

The prepared chitosan/pEGFP-TGF-β1 nanoparticles were spherical. Their particle size and Zeta potential were highly correlated with pH value. With the increase of pH value, the particle size increased and the Zeta potential decreased. The nanoparticles could effectively protect pDNA from degradation against nucleases. The encapsulation rate of pEGFP-TGF-β1 was (87.5±2.3)%; and pEGFP-TGF-β1 could be slowly released from the nanoparticles. Gene transfection in vitro proved that chitosan/pEGFP-TGF-β1 nanoparticles were efficient in transfecting chondrocytes and the expression of green fluorescent proteins was observed. Chitosan/pEGFP-TGF-β1 nanoparticles can effectively protect pDNA from nuclease degradation, have controlled release ability of TGF-β1 gene, and can mediate gene transfection against chondrocytes.

RESULTS AND CONCLUSION:

Lu HD, Lü LL, Zhao HQ, Wang K. Preparation and examination in vitro of transforming growth factor beta 1 gene releasing chitosan nanoparticles. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2120-2124. [http://www.crter.cnhttp://en.zglckf.com]

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