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Abstract

BACKGROUND:

The gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.

OBJECTIVE:

To evaluate the growth inhibition and heat killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugate on human laryngeal cancer Hep-2 cells under near-infrared hyperthermia.

METHODS:

The Hep-2 cells in the logarithmic growth phase were collected and divided into blank control group, irradiation group, gold nanochain irradiation group and anti-EGFR/Au irradiation group. After added with different culture medium, the near-infrared laser irradiation was performed in each group. Then the apoptosis of Hep-2 cells in different experimental groups was observed after the near-infrared laser irradiation.

RESULTS AND CONCLUSION:

Inverted microscope observation showed that the Hep-2 cells in the blank control and irradiation groups were still active and the Hep-2 cells in the gold nanochain irradiation group were damaged significantly, while the Hep-2 cells in anti-EGFR/Au irradiation group were damaged more serious. Flow cytometry analysis showed that the Hep-2 cells in the gold nanochain irradiation and anti-EGFR/Au irradiation groups had a varying degree of apoptosis and the increasingly heavy damage with the time. The gold nanochain irradiation can kill Hep-2 cells and the anti-EGFR/Au irradiation has a better effect in a time-depended manner.

RESULTS AND CONCLUSION:

Cong LH, Peng SK, He XG, Yang YB. Apoptosis of human laryngeal cancer cells induced by gold nanochain and anti-epidermal growth factor receptor/Au conjugate under near-infrared hyperthermia. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2145-2148. [http://www.crter.cnhttp://en.zglckf.com]

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