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Abstract

BACKGROUND:

Up to now, allograft vascular transplantation has failed in clinical application, as antigenicity rejection of allogeneic tissues is unsolved.

OBJECTIVE:

To prepare acellular vascular matrix materials and to assess the feasibility of acellular vascular matrix materials used for allograft transplantation.

METHODS:

A five-step process, including hypotonic, hypertonic solutions, 0.125% trypsogen, 1% Triton X-100 and 1% SDS detergents, was performed in the carotid arteries from three dogs. Histological examination and general tensile tests were done on the samples. And the acellular matrix allografts were transplanted into another five dogs.

RESULTS AND CONCLUSION:

The five-step process could remove all cells with the extracellular matrix well retained. The acellular matrix allografts (diameter of about 4 mm) were patent at explanting up to 10 months in dogs. The acellular arterial graft matrix was well prepared with the five-step process including detergents, such as Triton X-100, SDS and trypsogen without compromising the graft structure or mechanical properties significantly. And the carotid artery allografts decellularized by this process can be used directly in allogeneic transplantation, which are ideal to vascular transplantation.

RESULTS AND CONCLUSION:

Fan HH, Wu J, Zhang BX, Chen MZ, Zhang XH, Zhu KS. Acellular vascular matrix materials for allogeneic transplantation: A 10-month study. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2149-2152. [http://www.crter.cnhttp://en.zglckf.com]

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