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Abstract

BACKGROUND:

Silk fibroin and chitosan are non-toxic and have a good histocompatibility. However, they could not satisfy the biological scaffold material needs if working separately.

OBJECTIVE:

To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan, and to select the ideal scaffolds that suitable for the growth of osteoblasts.

METHODS:

2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2:C2H5OH:H2O= 1:2:8, filtration, concentration and purification. The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution. The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials. Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope; the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy, X-ray diffraction and electron spectroscopy.

RESULTS AND CONCLUSIONS:

More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan. The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts.

RESULTS AND CONCLUSIONS:

Liu L, Zeng SG, Ren L, Wei LQ, Yang J, Cao YN, Shi Y. Preparation and characterization of porous silk fibroin/chitosan scaffolds. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2197-2202. [http://www.crter.cnhttp://en.zglckf.com]

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