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Silk fibroin and chitosan are non-toxic and have a good histocompatibility. However, they could not satisfy the biological scaffold material needs if working separately.


To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan, and to select the ideal scaffolds that suitable for the growth of osteoblasts.


2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2:C2H5OH:H2O= 1:2:8, filtration, concentration and purification. The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution. The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials. Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope; the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy, X-ray diffraction and electron spectroscopy.


More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan. The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts.


Liu L, Zeng SG, Ren L, Wei LQ, Yang J, Cao YN, Shi Y. Preparation and characterization of porous silk fibroin/chitosan scaffolds. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(12): 2197-2202. [http://www.crter.cn]

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