SymbolTransfection of early growth response gene-1 DNA enzyme mediated by nanoparticles inhibits vein graft intimal hyperplasia

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Abstract

BACKGROUND:

The method of gene has been admitted to prevent stenosis and occlusion of grafted vascular. But it is a hot spot of how to increase gene transfection efficiency.

OBJECTIVE:

To study the effect of partial transfection of exogenous early growth response gene-1 DNA enzyme (Egr-1 DNA enzyme, EDRz) mediated by nanoparticles (NP) on the intimal hyperplasia (IH) in grafted vein.

METHODS:

The EDRz was constructed. Nanoparticle EDRz complex was prepared with polylactic/poly glycolic acid (PLGA) and polyvinyl alcohol (PVA). Autogenously vein graft model was established, and the 210 rats were divided into three groups randomly: (1) EDRz group, EDRz mediated by NP was transfected into the veins before anastomosis. (2) Empty vector group, the vein was transfected by empty vector mediated by NP. (3) Control group, no transfection.

RESULTS AND CONCLUSION:

In EDRz group, the expression of mRNA and protein of Egr-1 gene in intimal was significant decreased than that in other two groups (P < 0.05) and at the 7th, 14th and 28th days, the thickness of intimal hyperplasia was decreased than that in other two groups (P < 0.01), the apoptotic percentage of vascular smooth muscle cells in EDRz group was significant increased than that in other two groups (P < 0.05). EDRz expression can prevent intimal hyperplasia and promote apoptosis of vascular smooth muscle cells after autogenous vein grafting.

RESULTS AND CONCLUSION:

Liu CW, Zhang XS, Wang S, Wang SQ, Tian H, Cheng MX, Hu XH, Xin SJ, Duan ZQ. Transfection of early growth response gene-1 DNA enzyme mediated by nanoparticles inhibits vein graft intimal hyperplasia. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1354-1358. [http://www.crter.cnhttp://en.zglckf.com]

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