SymbolComponent analysis of a natural acellular sciatic nerve scaffold

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Currently, there are so many acellular methods used in the peripheral nerve, and two common methods are chemical extraction and frozen-thawing method. However, chemical extraction has the disadvantage of time-consuming and frozen-thawing method cannot achieve complete cell-free. Freeze-thaw+chemical extraction+mechanical vibration were chosen as optimization method for acellular processing of sciatic nerve in this study based on a large amount of literatures and repeated practice.


To analyze the morphology and composition of the natural acellular sciatic nerve scaffold prepared by optimization method.


Bilateral sciatic nerves from Wistar rats were randomly divided into two groups. The control group did not receive special treatment. The experimental group was treated with freeze-thaw+chemical extraction+mechanical vibration to prepare acellular scaffold.


Schwann cells, myelin sheaths and axons were removed completely from the optimized acellular sciatic nerve scaffold. And the scaffold showed a three-dimensional tubular structure. Extracellular matrixes of type I collagen, type III collagen and laminin were retained.


Cao RL, Li CY, Zhang XT. Component analysis of a natural acellular sciatic nerve scaffold.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1367-1370. [http://www.crter.cn]

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