SymbolComponent analysis of a natural acellular sciatic nerve scaffold

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Abstract

BACKGROUND:

Currently, there are so many acellular methods used in the peripheral nerve, and two common methods are chemical extraction and frozen-thawing method. However, chemical extraction has the disadvantage of time-consuming and frozen-thawing method cannot achieve complete cell-free. Freeze-thaw+chemical extraction+mechanical vibration were chosen as optimization method for acellular processing of sciatic nerve in this study based on a large amount of literatures and repeated practice.

OBJECTIVE:

To analyze the morphology and composition of the natural acellular sciatic nerve scaffold prepared by optimization method.

METHODS:

Bilateral sciatic nerves from Wistar rats were randomly divided into two groups. The control group did not receive special treatment. The experimental group was treated with freeze-thaw+chemical extraction+mechanical vibration to prepare acellular scaffold.

RESULTS AND CONCLUSION:

Schwann cells, myelin sheaths and axons were removed completely from the optimized acellular sciatic nerve scaffold. And the scaffold showed a three-dimensional tubular structure. Extracellular matrixes of type I collagen, type III collagen and laminin were retained.

RESULTS AND CONCLUSION:

Cao RL, Li CY, Zhang XT. Component analysis of a natural acellular sciatic nerve scaffold.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1367-1370. [http://www.crter.cnhttp://en.zglckf.com]

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