SymbolBiocompatibility of poly-L-lactic acid/chitosan electrospun nanofiber scaffold and rabbit endothelial progenitor cells

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Abstract

BACKGROUND:

Three-dimensional nanofiber scaffold, similar to extracellular matrix, can be synthesized from many polymer materials by electrospinning technique. Poly-L-lactic acid (PLLA)/chitosan (CS) nanofiber composite scaffold can overcome the deficiency of each other to improve the biocompatibility of tissue engineering scaffold.

OBJECTIVE:

To evaluate biocompatibility of PLLA/CS electrospun nanofiber scaffold and rabbit endothelial progenitor cells (EPCs).

METHODS:

Nanofibrou scaffold of PLLA, CS and PLLA/CS were synthesized by electrospinning technology and morphology structure was analysed by scanning electron microscope. After EPCs co-cultured with the nanofibers scaffold, adhere rates, NO production, morphological difference were investigated. Furthermore, phenotype of cell seeded on PLLA/CS was observed by immunofluorescence.

RESULTS AND CONCLUSION:

PLLA/CS nanofibrous scaffolds have better diameter of nanometer fiber than the PLLA and CS. Three-dimensional porous nanofibrous structure similar to extracellular matrix was founded in PLLA/CS nanofibrous scaffolds. Adherence rate and NO production of EPCs were improved by PLLA/CS nanofibrous scaffolds (P < 0.05, P < 0.01 ). EPCs could fuse with maintaining normal shapes and differentiation function in composite materials films of PLLA/CS. Cells in PLLA/CS expressed vWF and then exhibited endothelial characteristic. An excellent biocompatibility in PLLA/CS electrospun nanofiber scaffold and rabbit endothelial progenitor cells.

RESULTS AND CONCLUSION:

Wang W, Li K. Biocompatibility of poly-L-lactic acid/chitosan electrospun nanofiber scaffold and rabbit endothelial progenitor cells.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1396-1400. [http://www.crter.cnhttp://en.zglckf.com]

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