SymbolBiocompatibility between polylactic acid/chitosan nano-fiber/nano-hydroxyapatite scaffolds and rabbit chondrocytes

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Traditional scaffold materials have the disadvantages of strong hydrophobic property, lack of bioactive molecules for specific binding of cell surface receptors on their surfaces, easy to lead to aseptic inflammation by their acidic degradation products, and so on. The ideal scaffolds for cartilage tissue engineering can be prepared based on bionic principle and real structure and composition of cartilage.


To prepare the polylactic acid/chitosan nano-fiber/nano-hydroxyapatite (PLLA/CSNF/nHA) scaffolds, evaluate their biocompatibility rabbit knee chondrocytes, and explore their application in articular cartilage tissue engineering.


The PLLA/CSNF/nHA scaffolds were prepared by two steps separation technology. Chondrocytes from New Zealand rabbits were seeded on the scaffolds for compound culture. The cell growth was observed under inverted phase contrast microscope. Cell-scaffold compounds were cultured in 24-well plates for 5 days, and then the compounds were implanted under the skin of nude mice for 8 weeks.


The prepared PLLA/CSNF/nHA scaffolds had an appropriate three-dimensional porous structure. The porosity was 90% and the pore size was 300-450 μm. Chondrocytes in cell-scaffold compounds could secrete proteoglycan and type II collagen like natural cartilage tested by immunohistochemical staining and toluidine blue staining. It is indicated that PLLA/CSNF/nHA has a good biocompatibility with rabbit chondrocytes and can be used as a type of bio-engineered stent.


Liang HZ, Zha ZG, Yao P, Wu H, Liu N, Li JR, Huan SW, Miao GQ, Zhao JH. Biocompatibility between polylactic acid/chitosan nano-fiber/nano-hydroxyapatite scaffolds and rabbit chondrocytes. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1413-1416. [http://www.crter.cn]

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