SymbolConstruction of artificial bioactive dermis using acellular amniotic membrane loaded with controlled-released basic fibroblast growth factor

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Abstract

BACKGROUND:

There are many kinds of skin tissue substitutes with their own advantages and disadvantages. However, no one is the perfect to apply in clinical practice.

OBJECTIVE:

To discuss the possibility of constructing a new kind of artificial bioactive dermis loaded with controlled-released basic fibroblast growth factor (bFGF).

METHODS:

Children foreskin fibroblasts were cultured with tissue method. Human acellular amniotic membrane was prepared by the enzyme-detergent method. Two-phase method was performed to prepare the sustained release microspheres of bFGF-gelatin-chitosan. The sustained release microspheres were adhered to the acellular amniotic membrane. The third and forth generations of fibroblasts were cultured on the acellular amniotic membrane loaded with sustained release microspheres.

RESULTS AND CONCLUSION:

The prepared acellular amniotic membrane was white and translucent film-like. It had a high porosity, and the pore size was irregular and varies from 10 to 100 nm. The surface of bFGF-gelatin-chitosan microspheres was spherical, in uniform size and smooth. The drug-loading rate and encapsulation efficiency of microspheres were 20 ng/g and 80.5% respectively. The fibroblasts showed a well creeping growth on the scaffolds, and the laminin was more than that of the control group. It is indicated that the fibroblasts can be cultured on the acellular amniotic membrane loaded with sustained release microspheres of bFGF-gelatin-chitosan, and the sustained release microspheres can be well adhered to the acellular amniotic membrane.

RESULTS AND CONCLUSION:

Wu RD, Tang S, Su AY, Tang Q. Construction of artificial bioactive dermis using acellular amniotic membrane loaded with controlled-released basic fibroblast growth factor. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1425-1429. [http://www.crter.cnhttp://en.zglckf.com]

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