SymbolEffects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of streptococcus mutans

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Abstract

BACKGROUND:

The preliminary study has confirmed the existence of cyclic dimeric guanosinemonophosphate (c-di-GMP) signaling pathway in streptococcus mutans. The pathway mediates the streptococcus mutans biofilm formation and in vitro adhesion to enamel surface.

OBJECTIVE:

To analysis the effects of c-di-GMP on the biological properties of streptococcus mutans by gene chip technology.

METHODS:

Exogenous c-di-GMP was used to interfer streptococcus mutans UA159. The total RNA was extracted, the total RNA of standard strain was as control, and then hybridized with streptococcus mutans whole-genome chips. The different genes were filtered.

RESULTS AND CONCLUSION:

Expressions of 9 genes (glgA, glgB, msmF, gftA, lacG, lepB, rgpAc, bacC, apt6) were up-regulated while six genes (Hrc, ProC, HprT, Pdp, Glk, cdsA) expressions were down-regulated after exogenous c-di-GMP interfering. Differences genes were mainly related to the biological characteristics, such as cell tropism, biofilm formation, signal transduction, and energy metabolism. It showed that the c-di-GMP could affect the cariogenic ability of streptococcus mutans.

RESULTS AND CONCLUSION:

Yan WJ, Xu SJ, Xie MM, Wu BL. Effects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of streptococcus mutans. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(8): 1451-1454. [http://www.crter.cnhttp://en.zglckf.com]

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