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Abstract

BACKGROUND:

Hydrostatic pressure stimulation caused by gravity is an important regulator of human nucleus pulposus cells metabolism.

OBJECTIVE:

To observe the effect of hydrostatic pressure on the morphology of monolayer cultured human nucleus pulposus cells and matrix expression in vitro.

METHODS:

In the hydrostatic pressure loading system, 0.3, 0.7, 3 MPa pressure were imposed on the passage 4 human nucleus pulposus cells in monolayer in vitro culture for 30, 60, 90, 120 minutes, and the atmospheric pressure 0.1 MPa group was as the control group.

RESULTS AND CONCLUSION:

①Morphology: cell volume became small under hydrostatic pressure stimulation. Under 0.3 and 0.7 MPa hydrostatic pressure, cell volume shrinked slightly, and cell morphology were relatively complete. Under 3 MPa hydrostatic pressure, cell volume was significantly reduced, and cell morphology was not complete. ②Survival rate: after 30 minutes hydrostatic pressure stimulation, survival rate were relatively low in all groups, as time passed, the rate gradually increased or remained stably in 0.3, 0.7 MPa groups, and tended to decline in 3 MPa group.③ Proteoglycan expression: the expression were increasing with time in all groups, and when hydrostatic pressure sustained for 120 minutes, the strongest proteoglycan expression were detected in 0.3 and 0.7 MPa group which were significantly more than that in the 0.1 and 3 MPa group. The results show that hydrostatic pressure would affect nucleus pulposus cell morphology, viability and proteoglycan expression.

RESULTS AND CONCLUSION:

Chen J, Liu GZ, Xu L, Li CG, Sun Q, Qiao WP, Zhu ZQ. Effect of hydrostatic pressure on the morphology and matrix expression in human nucleus pulposus cells.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(13): 2281-2286. [http://www.crter.cnhttp://en.zglckf.com]

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